HPLC analysis of methanolic extract of herbs for quercetin content

Abstract

Herbs are rich source of flavonoids. Flavonoids are polyphenolic compounds that are ubiquitous in nature and are categorized according to their chemical structure, into flavones, isoflavonoes and flavon-3-ol, anthocyanidins. They have aroused considerable interest because of their potential beneficial effect on human health. They have been reported to have antiviral, anti allergic, anti platelet, anti inflammatory, antitumor, anti carcinogenic and anti ageing properties. HPLC analysis can be used for classification of herbs based upon secondary metabolites. Extract yield at optimum condition was then analyzed by high performance liquid chromatography (HPLC) for quantifying bioactive flavonoid compounds. It was observed that mint contains the highest concentration of quercetin i.e. about 10.8mg/g.