Antitumor activity of <em>Annona muricata</em> L. fruit and leaves in Ehrlich's ascites carcinoma cell- treated mice

Abstract

Purpose: The main goal of this study was aimed to evaluate the antitumor effect of A. muricata extract of both leaves and fruit in EAC-bearing mice model in comparison with a standard therapeutic agent, cisplatin. Methods: The antitumor activity of the ethanol extracts of Annona muricata were evaluated against Ehrlich ascites carcinoma (EAC) tumor in mice at dose of 200mg/kg body weight orally. Mice were divided into five groups. Group I: (Normal group) mice were received (0.2 ml Saline), Group II: (Cancer group) 20 mice were intraperitoneally received EAC cells (2×106cells/mouse I.P.), Group III: (EAC + leaves extract) 20 mice were orally treated after 48 hours from injection of EAC cell with a dose of 200mg/kg body weight daily for 9 consecutive days, Group IV: (EAC + fruit extract) mice were orally treated after 48 hours from injection of EAC cell with a dose of 200mg/kg body weight daily for 9 consecutive days, Group V: (EAC+ cisplatin), 20 mice were intraperitoneally treated with cisplatin after 48 hours from injection with EAC at a daily dose of 2 mg/kg body weight for 9 consecutive days. Total experimental period was 11 days, after 24 h from the last dose, 8 mice in each group were anesthetized by diethyl ether and sacrificed for the histopathological examination of Kidney tissue.Results: Administration of the extracts resulted in that A. muricata has protective role on the management of histopathological changes occurred in cancer induced mice.Conclusion: The results suggest that the ethanol extract of Annona muricata L. Leaves and fruit showed anticancer effect when compared to the tumor group.