Genetic diversity of bread wheat genotypes ( Triticum aestivum L.) revealed by microsatellite SSR markers for leaf and stripe rust resistance

Abstract

The present investigation was conducted to evaluate 40 diverse genotypes of bread wheat and to characterize these genotypes using Simple Sequence Repeat (SSR) markers. A total of 27 SSR primers were used in study. Three SSRs (Xgwm 582, LrK10-1AS and gwm344) did not show amplification of genomic DNA from different wheat genotypes. Only 24 SSRs (88.8%) showed amplification, of which 11 SSRs (40.7%) produced polymorphic bands while 13 SSRs produced monomorphic bands (44.4%). Size of amplified product in different genotypes ranged from 150-400 bp, a total of 44 alleles were detected with 1.83 as the average number of alleles detected.