Isolation and characterization of urolithin b from asphaltum

Abstract

Asphaltum (vernacular name: Shilajit), is a traditional Ayurvedic medicine used as aphrodisiac. The present study was aimed for extraction, isolation, method development and validation of RP-HPLC method for bioactive compound called urolithin B (benzocoumarin) for Asphaltum. HPLC method was developed with the help of PDA detector for isolated compound. Method validation was performed as per International Conference of Harmonization guidelines for linearity, accuracy, precision, specificity, limit of detection and limit of quantification of urolithin B. Intra-day and Inter-day precisions for urolithin B was found precise as ≤ 2.0% in intraday exercise & ≤ 3.0% in inter day exercise. The concentration found in Asphaltum is 0.38% of urolithin B. The proposed method for estimation of urolithin B is found to be accurate, precise, reproducible and repeatable.