Beauveria bassiana and Metarhizium anisopliae in vitro germination in culture media supplemented with the phases of the development of Aedes aegypti

Abstract

In this study was to evaluate the conidial germination of EF strains: Metarhizium anisopliae (1 strain) and Beauveria bassiana (2 strains) in two culture media (Agarose and Sabouraud Dextrose Agar [SDA]) supplemented with eggs, larvae, pupae and adults of Aedes aegypti vector. M. anisopliae 028 presented the highest germination on Agarose media supplemented with larvae or pupae (99% and 98% at 22 hours) and (97% and 98% at 12 hours) on SDA media supplemented with larvae or adults, B. bassiana 120 presented the highest germination percentages on the SDA media, as on Agarose (99% and 98% in 12 and 20 hours respectively) supplemented with larvae or pupae. Respect to B. bassiana 103 strain by the vector addition to the culture medium, no effect was observed. The results indicate that M. anisopliae 028 and B. bassiana 120 strains are Ae. aegypti potentials biocontrollers agents.