Effect of Indian spices on lasR mutant of Pseudomonas aeruginosa PAO1 biofilm

Abstract

The study was undertaken to analyse the impact of bark of cinnamon (Cinnamomum zeylanicum), seeds of mustard (Brassica nigra) and nutmeg fruit (Myristica fragrans) on the biofilm by lasR mutant of Pseudomonas aeruginosa PAO1. The analysis of the phytoextracts showed that the test culture is inhibited to varying degrees by different phytoextracts. Aqueous and methanolic extracts were prepared and its phenolic content and antioxidant properties were assessed. The effect of the extract was further studied for the biofilm formation by the lasR mutant. Different phytoextract cause varying degree of structural changes in biofilm architecture of wild type and lasR mutant. The finding indicates involvement of lasR during biofilm development in presence any antimicrobial compound of plant origin.