In vitro antidiabetic assessment of Ocimum forskolei L growing in Saudi Arabia

Abstract

The main objective of current study was to investigate the in vitro antidiabetic activity of Ocimum forskolei‎.In vitro antidiabetic assays such as inhibition of α-amylase enzyme, non-enzymatic glycosylation of hemoglobin were carried out.The results of α- amylase inhibition assay revealed that the inhibitory activity (IC50) of both leaves and stems methanol extracts are almost the same (‎72.3 and ‎78.9µg/ml, for leaves and stems extracts, respectively) when compared with positive control (Acarbose IC50 value of ‎45.3‎ µg/m). Regarding the inhibition of glycosylation of hemoglobin, inhibitory activity (IC50)both leaves and stems methanol extracts ‎ were‎ (‎54.4 and‏ ‏‎70.3µg/ml, for leaves and stems extracts, respectively) when compared the positive control, alpha-tocopherol (‎35.4µg/ml‎). The results from this study indicated that Ocimum forskolei ‎ leaves and stems methanol extracts showed considerable in vitro antidiabetic activity.