Identification of the SSR marker linked to powdery mildew resistant gene(s) in Brassica spp.

Abstract

The present investigation was carried out to obtain information on screening of molecular markers for powdery mildew resistance in Indian mustard. The molecular analysis for powdery mildew resistance in brassica spp. was carried out with four females, three males, F1S and F2S generation. The experimental materials were evaluated in randomized block design with three replications. The SSR markers viz, OI10-B12 and OI10-C01 clearly distinguished between susceptible and resistant bulks of interspecific cross GM-3 x PusaSwarnim. However, these markers can also be utilized for identifying disease resistance in other brassica species also, since over the years the ‘C’ genome introgression to other Brassica genotypes may have occurred through natural out crossing. But disease resistant reaction of genotypes will greatly depend upon the congenial environment prevailing for diseases outbreak. Therefore the markers identified in this study may be influenced by the prevailing environmental and the eco-geographical factors which need to be taken into consideration while attending further phenotypic screening for disease resistance and validating with the molecular markers.