An evaluation of the geographical influence on the α-amylase bioactivity of actinomycetes - isolated from Coringa Mangroves

Abstract

Mangroves are the versatile and indomitable plant community for a wide range of bioactivities owing to its unique marine - terrestrial extreme interface. The objective of the current study is to evaluate the hypothesis, to what extent the geography (distance from the shore) influences the α-Amylase production from mangrovian actinomycetes. Six different soil samples (A1, A2, A3, A4, A5 and A6) were collected from Coringa mangroves based on shore to distant shore and were subjected to serial dilution and isolation techniques. A total of nine strains were identified based on colony characteristics. The primary screening for α Amylase recognized two strains (ANUKCJV1:132135 – A5 and ANUKCJV2:130305 – A2) as the potential candidate strains and were subjected to α-Amylase production, the two strains exhibited 0.491 and 0.303 IU of α-Amylase activity. The two potential strains were identified as Streptomyces pluripotens and Streptomyces chilikensis respectively based on 16S r DNA sequencing. The sequences of the two potential strains were deposited in the NCBI with accession codes; ANUKCJV1 – MH973320 and ANUKCJV2 – MH973321 respectively. Based on the current study it may be concluded that both the extremeness and favourability of the mangrovian environ influences the α-Amylase production.