Antioxidant, antimicrobial and cytoprotective action of ethanolic extract of Glycyrrhiza glabra root against ccl4 induced damage on Saccharomyces cerevisiae

Abstract

Ethanolic extract of Glycyrrhiza glabra root was investigated with antioxidant, antimicrobial and Cytoprotection assays in different in vitro models. Diphenyl picrylhydrazyl (DPPH) radical, inhibition of Reactive oxygen species (ROS), Lipid peroxidation (LPO), Reducing power, total phenolic content were some of the antioxidant assays performed. From the results obtained it was observed that the DPPH radical activity and ROS showed IC50 of 55 and 20µg respectively and LPO with IC50 of 6 µg. The extract also showed good reducing activity, which is an index of hydrogen donor. Total phenolic content was recorded 100mg/g of the extract. Further the extract showed cytoprotective effect on xenobiotic induced (CCl4) oxidative stress in Saccharomyces cerevisiae (yeast cells). Cell viability, ROS, TBARs, LDH leakage in yeast cells were studied. From the obtained results it shows that the ethanolic extract of Glycyrrhiza glabra is having potent radical scavenging molecules which minimized the damage and protected cell, which was evident with decrease in the level of ROS, LPO and LDH leakage. The extract also showed antimicrobial activity with inhibition in growth of different Microorganism, B. cereus, S. aureus, and E. Aerogens and E. coli. The broad range of activity of the extract suggests that Glycyrrhiza glabra is a source of natural antioxidant, antimicrobial and cytoprotective, which could be considered as nutraceutical with health promoting properties in the prevention, and amelioration of degenerative diseases.