An In-Vitro antibacterial effect of Momordica foetida and Croton macrostachyus on Streptococcus agalactiae Isolated from bovine mastitis

Abstract

Two plants, Momordica foetida and Croton macrostachyus were collected from South Nation Nationality of People Region from Kembata Tembaro Zone specially Durame area. In-vitro antimicrobial sensitivity tests of both plants were conducted in Addis Ababa University College of Veterinary Medicine and Agriculture, Debre Zeit, from October 2016 to April 2017. The study was carried out with the objective of determining and comparing of the in-vitro antimicrobial effects of root of Momordica foetida and bark of Croton macrostachyus on three isolates of Streptococcus agalactiae coming from bovine mastitis cases. In this study, the 80% methanol crude extract preparation of root of Momordica foetida and bark of Croton macrostachyus had good antibacterial activity against the tested bacteria. The extracts of the plants inhibited the growth of Streptococcus agalactiae at all concentrations (0.625%, 1.25%, 2.5%, 5%, and 10%). In all cases, there was a dose dependent inhibition on the tested bacteria. Conventional antibiotic disc and DMSO (Dimethyl sulfoxide) impregnated discs were used as positive and negative controls, respectively and no inhibition was observed by DMSO which substantiates that the inhibition observed was exclusively by crude extracts. This finding suggests that there is a promise in combating drug resistant pathogens.