Role of circulation miRNA in patients suffering with polycystic ovary syndrome (PCOS)

Abstract

Introduction: Polycystic ovary syndrome (PCOS) is a hormonal disorder common among women of reproductive age. Women with PCOS may have infrequent or prolonged menstrual periods or excess male hormone (androgen) levels. Material & Methods: A total of 110 patients with PCOS and 125 healthy controls were included in the present study. Total miRNA were isolated for 4mL of blood samples followed by cDNA construction. Furthermore, selected miRNAs (miR-21, miR-146a, miR-200a, miR-92a & miR-92b) were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Results: In the present study, we demonstrated that women with PCOS had significantly increased expression level of miRNA 21 and miRNA146 compared to healthy controls. Our study revealed that the expression level of miR‑92a, miR‑19b and miR‑200a were significantly down regulated in patients with PCOS compared with controls. Conclusions: miRNAs are differentially expressed between PCOS patients and controls. We identified and validated five different miRNAs. They are significantly up and down regulated and may be involved in the pathogenesis of PCOS.