Anti-thrombotic activity of isolated β-sitosterol from roots of Hemidesmus indicus Linn in rat model

Abstract

Thrombosis is the basic pathophysiological procedure, brings about majority strokes and heart attacks which are serious causes of death all over the world. The present work was aimed to study the antithrombotic activity of isolated compound -sitosterol (BS) from roots of herb Anantamul (Hemidesmus indicus Linn). A number of engrossing biomedical properties have been attributed to BS. Antithrombotic activity was evaluated by in-vitro analysis of different experimental paradigm i.e. Free radical scavenging activity, Clot lysis assay, Malondialdehyde (MDA) production in blood platelets and in-vivo models such as Rat arterio-venous shunt silk thread model, Rat venous thrombosis model, Carrageenan induced thrombosis. The statistical results obtained from the experimental models inferred that isolated BS imputed to its anti-thrombotic activity due to the anti-oxidant property, clot lysis property, inhibition of lipid peroxidation in platelet, inhibition of platelet lysis and effect on coagulation cascade.