articleTop 1% cited

Single-pot, solid-phase-enhanced sample preparation for proteomics experiments

Nature Protocols · 2018 · Vol. 14(1) · pp. 68–85

Christopher S. Hughes✉(Canada's Michael Smith Genome Sciences Centre)Sophie Moggridge(University of Victoria)Torsten Müller(German Cancer Research Center)Poul H. SorensenGregg B. Morin(University of British Columbia)Jeroen Krijgsveld(German Cancer Research Center)

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Advanced Proteomics Techniques and Applications Mass Spectrometry Techniques and Applications Protein purification and stabilityChaotropic agentSample preparationChromatographyProteomicsLysisProtein purificationReagentChemistryComputer scienceMaterials science

MeSH terms

BuffersDetergentsChemical FractionationHumansSolventsSpecimen HandlingUreaIsothiocyanatesGuanidineProteomeProteomicsSolid Phase MicroextractionHEK293 CellsHydrophobic and Hydrophilic Interactions

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References

Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells