Standardization of callus induction protocol and effect of hormone concentration on synthesis of Andrographolide from Andrographis paniculata

Abstract

Andrographis paniculata is commonly known as Kalmegh or Kirayat. Belongs to Acanthaceae family and annual herbaceous plant found in Asian countries like India and Sri Lanka. It has high market demand because of its various medicinal uses like treating dysentery, cholera, diabetes, influenza, etc. Andrographolide is major secondary metabolite which is present in Andrographis paniculata. It shows medicinal properties like Anti-Cancer, Anti-inflammatory, Anti-Venom, etc. Andrographolide is majorly found in leaves of Andrographis paniculata. In this present study we have standardize protocol for callus induction by using growth hormones like BAP, NAA and 2,4-D with different combinations. Excellent quality of callus were used for HPLC analysis. Results revealed that as concentration of hormone enhances content of Andrographolide also enhances and its vice versa. Major content was found 2.36% and lowest was 0.92%. Percentage of content was calculated with the help of formula.