Genetic purity test of hybrid rice (Oryza sativa L.) based on chemical test

Abstract

The present investigation was carried out to characterize with one seed (female) parent and ten pollen parents and their ten hybrid combinations using chemical tests for genetic purity testing. The seeds were subjected to phenol and NaOH test for differentiating the parents and their hybrid. Out of two chemical tests only phenol test was able to differentiate between the parents and their hybrid. The hybrids having distinct colours in phenol test namely IR68888A/Kapilee and IR68888A/Teraboli with brown colouration and IR68888A/Luit with light brown colouration could be distinguished from their parents. However, the NAOH test did not differentiate between the parents and their hybrid. All the genotypes and hybrid were showed same colour reaction.