Fractionationation of purified terpenoids from red algae Hypnea musciformis (Wulfen) J.V. Lamouroux. and Kappaphycus alvarezii (Doty) Doty Ex P.C. Silva. By Gc: Ms analysis

Abstract

Terpenoids represent the earliest biomolecules, having been reported from earth sediments from 2.5 billion years ago. Most common phytochemical in the plants that shows high diversity. This feature in turn reflects their unique biological activities and there by forms the resource for traditional human exploitation. In this study the terpenes composition from the red algae Hypnea musciformis and Kappaphycus alvarezii were identified and estimated by using Gas Chromatography- Mass Spectrum. The crude methanolic extract of Hypnea musciformis and Kappaphycus alvarezii were purified by Column Chromatography. The analysis of the purified fraction of Hypnea musciformis and Kappaphycus alvarezii revealed the presence of 8 major peaks and 12 major peaks of terpenoids respectively. Significant differences were found in the terpenes composition in both algae. The total number of terpenoid composition was found to be highest in Kappaphycus alvarezii. The main terpene component was the Hexadecanoic acid, methyl ester in Hypnea musciformis where as in β-amyrin in Kappaphycus alvarezii as per the concentration percentage.