Study of the chlorination of guanosine monophosphate by hypochlorous acid using a rotating platinum electrode

Abstract

The in vitro chlorination of the nucleotide Guanosine Monophospate (GMP) by Hypochlorous acid (HOCl) has been herein studied. The in vivo chlorination of nucleobases and nucleotides gives rise to mismatch pairing in genetic coding which in turn results in abnormal protein synthesis and consequently diseases. The reaction studied is found to be rapid and the use of the special kinetic technique - hydrodynamic voltammetry has been adopted. The chlorination results in the formation of the mono-chloro product at position 8 on the substrate nucleotide.