Biochemical and histopathological changes in the treatment of chronic cutaneous wound in New Zealand white rabbits with bio-ceramic device

Abstract

In this study, 18 (eighteen) numbers of adult rabbits of either sex of New Zealand White maintained under proper managerial condition in the Department of Surgery & Radiology, College of Veterinary Science, AAU, Khanapara were used to produce surgical wounds. The animals were randomly divided into three (3) groups i.e. A, B and C with each group consisting of six (6) animals. The biochemical changes were recorded on 3rd, 6th and 9th day but the histopathological changes were recorded on 9th day. The surgical sites at the thoraco-lumber region were prepared by clipping and application of antiseptic solution (Povidone iodine).The Protein content, Alkaline phosphatase, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Creatine kinase (CK) were determined and estimated by collecting 50 mg of wet tissue from each wound area. The biopsy materials from the wounds of 4mm thickness on 9th day post wound creation were collected and preserved in normal buffered formalin. The maximum protein level of wound tissue homogenate was recorded on 9th day of experiment in all the groups. There was an initial rise of alkaline phosphatase activity in wound tissue homogenate on 3rd day in all the groups. There was initial rise of creatine kinase activity in the wound tissue homogenate in all the groups on 3rd day observation. Thereafter, the creatine kinase activity in the wound tissue homogenate was declining towards the end of the experiment. The histopathological examination of the healing tissue taken on 9th day in Group B revealed thin layer of epidermal cells with a thin keratin layer without any scab. In Group A, some scab materials were still present on the wound surface on 9th day with a thin layer of squamous epithelial cells covering the wound surface. The data were analyzed as per methods described by Snedecor and Cochran (1994) and were presented accordingly.