Evalution of therapeutic efficacy of Nigella sativa and Azadaricta indica in subclinical mastitis in buffaloes

Abstract

For the study a total of 24 buffaloes suffering for subclinical mastitis were randomly divided into four groups comprising of 6 animals in each group, besides this, six animals were kept as healthy control (Group-A). Under the experiment Azadirachta indica (Neem) sterilised methonolic seed extract @ 700mg/5ml phosphate buffer saline for 5 days and Nigella sativa (kalonji) sterilised methonolic seed extract @ 10ml 6% solution in liquid paraffin for 5 days were used intramammary as well as topically in different groups. Amongst the various groups under therapy, the results revealed that group third in which Nigella sativa (intramammary and topically) was given found to be the best in respect of maximum number of animals recovered (5/6) on 7th, 15th and 30th days post treatment with significantly decreased milk pH (6.85 ± 0.23*) and decreased Somatic Cell Count (3.21 ± 0.76*x 105 cells/ml) with better clinical recovery.