Phytochemical analysis of ethyl acetate extract of Tinospora cordifolia by mass spectrometry

Abstract

The present study was carried out to investigate the presence of phytochemical constituents in ethyl acetate extract of Tinospora cordifolia stem using GC-MS. In the GC-MS analysis, 45 numbers of phytochemicals were identified. Out of the 45 compounds obtained, 09 compounds had the higher matching probability above 40% with the standard compounds of the NIST Library database namely (E)-4-(3-Hydroxyprop-1-en-1-yl)-2-methoxyphenol (47.67%); l-(+)-Ascorbic acid 2,6-dihexadecanoate (59.49%); Octadecanoic acid (65.96 %); Columbin (1,4-Etheno-3H,7H-benzo[1,2-c:3,4-c']dipyran-3,7-dione, 9-(3-furanyl) decahydro-4-hydroxy-4a,10a-dimethyl-, [1R-(1a,4a,4aa,6aa,9a,10aa,10ba)]-) (83.88 %); Campesterol (Ergost-5-en-3-ol, (3a,24R)-) (65.36 %); Stigmasterol (Stigmasta-5,22-dien-3-ol, (3a,22E)-) (62.76 %); c-Sitosterol (Stigmast-5-en-3-ol, (3a,24S)-) (85.21 %); 9-Ethyl-3-hydroxy-10,11b-dimethyl-1,2,3,4,6,6a,6b,7,8,9,11a,11b-dodecahydrobenzo[a]fluoren-11-one (53.57 %) and a-Amyrin (Olean-12-en-3-ol, (3a)-) (49.54%). Presence of various phytochemicals indicates the antioxidant, antimicrobial, antiviral, antinflammatory, antidiabitic, analgesic, hepatoprotective and flavouring agent property of Tinospora cordifolia. Hence, the presence of phytochemicals reported in the present study indicates that the Tinospora cordifolia could serve as “herbal feed additive” in poultry industry in the coming years.