Tissue distribution of the antigenic variants of canine parvovirus in various organs using nested PCR

Abstract

Canine parvovirus-2(CPV-2) is a highly contagious and fatal disease of dogs causing acute haemorrhagic enteritis and myocarditis. In this study, tissue samples such as brain, cerebellum, cerebral bulb, tonsil, retropharyngeal lymph node, thymus, lungs, myocardium, bone-marrow, liver, spleen, pancreas, kidney, bladder, mesenteric lymph node, stomach, duodenum, jejunum, colon and rectum collected from 3-9 months old dead dogs reported with severe clinical symptoms of CPV were subjected to conventional and nested PCR assay, CPV-DNA was detected in all tissues including brain tissue.