Biosynthesis of silver nanoparticles and their evaluation of antifungal activity against Magnaporthe oryzae

Abstract

Rice (Oryza sativa L) is the most important cereal grain and food stuff which forms an important part of the diet of more than three billion people around the world and popularly called as Global grain. Blast of rice caused by Pyricularia oryzae Cav. (Magnaporthe grisea Sacc.) is one of the most destructive disease and it accounts for 30 to 100% yield losses in all rice growing areas of the country. The blast of rice causes huge losses of quality and quantity of harvest. However the fungus became resistant to chemical fungicides and these causes a global pollution so an alternate method is needed to control the blast disease so we go for biologically synthesized silver nanoparticles to control the disease. Isolation of M.oryzae from the infected necrotic patches of diseased leaves in rice plant. After a week to prepare a spore suspension in 3 ml of water. And then prepare a seeded media of PDA using the M.oryzae and carry out of the Agar well method at a different concentration by using synthesized silver nanoparticles by B.vallismortis BVM4, L.macroides LBM7 and Tricyclozole (a chemical to control blast disease) at different concentrations. By this the maximum zone of inhibition was recorded at 3.0µg/ml (30mm) of B.vallismortis BVM4. The minimum zone of inhibition was observed of 0.1µg/ml (20mm) of tricyclozole