Best plant harmon combination for In vitro callus initiation, organogenesis and regeneration of rice cv. swarna sub1

Abstract

The rapid increase of population is not in tally with the increase of crop production which is leading the world to a severe food crisis. It has become the need-of-the-hour to increase the production of cereals by introducing traits like high yield, disease tolerance, submergence tolerance, etc. Mature de husked rice seed most suitably sterilized with HgCl2 (0.1%) treatment for 5 minutes and observed that media containing 2, 4-D (1.5 mg/l) + BAP (0.5 mg/l) supported maximum callus induction and a percentage of 80% was obtained which was highly organogenic also. When the Somatic embryo of Swarna Sub1 inoculated in MS media supplemented with NAA (0.5 mg/l) +BAP (3 mg/l) + KIN (0.5 mg/l) produces average 10 shoot and 4 root initiation. The protocols developed will facilitate large scale propagation of the transformed plants.