Electrophoretic pattern of serum protein in Streptozotocin induced diabetic rats treated with palm oil and Tinospora cordifolia leaf extract

Abstract

The present experiment was carried out to study the effect of palm oil and Tinospora cordifolia leaf extract to experimentally induced diabetic rats on serum electrophoretic pattern. 24 male Wister rats weighing 180-200g were divided into four groups of six animals in each group. Diabetes was induced by the administration of (40 mg / kg body weight) through intra-peritoneal route. The experimental rats were fed diet that had similar composition except for fat source, which consisted of 8% ghee and 8% palm oil. The first group served as control given diet containing 8% of ghee. The second group was diabetic group fed diet supplemented with ghee. The third group was diabetic and fed diet supplemented with palm oil and the fourth group was also diabetic and fed diet supplemented with ghee and treated with Tinospora cordifolia leaf extract. The experiment was carried out for 60 days. At the end of the experiment, the rats were sacrificed and blood was collected. Serum was separated and total protein and albumin were estimated. The serum protein level was significantly reduced and albumin concentrations were not significantly differed in all the treatment groups compared to control group. Electrophoretic pattern of serum proteins has revealed the absence of proteins having the molecular weight of 24.9KDa in diabetic and treatment groups as observed in control group.