In-vitro direct shoot organogenesis in Jatropha curcas L.

Abstract

An efficient and reproducible plant regeneration protocol was developed from axillary bud explants derived from physiologically mature trees of Jatropha curcas L., a promising tree for production of biodiesel. Direct organogenesis yields true to type plantlets. PVP (1.0 g/l), ascorbic acid (150 mg/l) and citric acid (50 mg/l) was used to effectively counter the phenolics released from the explants of the mature tree. The additives like adenine sulphate (25 mg/l) and glutamine (50 mg/l) helped to enhance the growth of Jatropha curcas explants. 91.65 ± 0.31 % shoot induction was observed in the axillary bud explants on MS medium supplemented with BA (2.00 mg/l) + Kinetin (1.00 mg/l) + GA3 (0.10 mg/l). The maximum number of shoots (10.24±0.07) was observed in the MS + BA (2.00 mg/l) + Kinetin (1.00 mg/l) + GA3 (0.05 mg/l). Maximum shoot length of 07.21 ± 0.25 was obtained on the MS medium supplemented with BA (2.00 mg/l) + Kinetin (0.50 mg/l) + GA3 (0.10 mg/l). Profuse rooting of the regenerated shoots were obtained from the on the MS medium supplemented with IBA (1.00 mg/l) + NAA (1.00 mg/l) having a root induction response of 89.67±0.23 %. The highest number of roots (06.46±0.28) was obtained on MS + IBA (1.50 mg/l) + NAA (0.10 mg/l) having root length (04.53±0.25 cm). The survival percentage of the plants in the field after acclimatization was 80.0%.