Conserving broodlac on Flemingia semialata in Albizia procera (siris) plantation during summer season under rainfed condition

Abstract

Micropropagation is a promising technique for development of pathogen and virus free microtubers which can be used as better “potato seed” than those produced conventionally. Genetic stability and biosafety of such seeds is matter of concern before its consumption. Genetic fidelity of micropropagated microtubers of potato (Solanum tuberosum), developed as in vitro cultured pathogen free “potato seeds”, was evaluated using Random amplified polymorphic DNA (RAPD) markers. Nine arbitrary decamers were used to amplify DNA from in vivo control tuber and in vitro micropropagated microtubers to assess the genetic fidelity. RAPD profiles from all the micropropagated microtubers were monomorphic and similar to those of field grown control tubers. There was no detectable variation within the micropropagated microtubers which suggests its genetic stability.