Regeneration potential of scutellum-derived calli from different Indica rice (Oryza Sativa L.) varieties

Abstract

The present investigation was carried out to standardize efficient and reliable regeneration protocol in scutellum-derived rice calli from 22 rice varieties developed by Dr. B.S.K.K.V., Dapoli. All the varieties with all the media combinations and their interactions significantly affected the regeneration potential of the calli. The variety Ratnagiri-711 showed earliest shoot induction (11.67 days) among the all on mediumMS +3.0 mg/l BAP +1.0 mg/l IAA showing highest shoot induction frequency (87.50%) and maximum number of shoots per callus (7.67). It rooted also earlier in 3.67 days after transferring the shoots to rooting medium 1/2 MS +0.5 mg/l IBA +0.1 mg/l BAP and shown highest root induction frequency (73.33%) with maximum roots per shoot (11.33). For hardening, potting mixture of soil, organic manure and cocopit in 1:1:1 proportion showed highest survival of In Vitro grown plantlets (73.33%). No morphogenesis was observed on only MS medium without growth regulators.