Quantitative analysis of Sinigrin in Brassica Juncea

Abstract

It has been reported that Brassica juncea (Indian mustard) contains significant amounts of aliphatic glucosinolate, sinigrin. Quantitative analysis of sinigrin was done in thirty genotypes of Brassica juncea. All the genotypes were grown in the field and samples of different plant tissues were collected from randomly selected plants for High performance liquid chromatography (HPLC) analysis. Significant variation was found among the genotypes as well as within the plant. Sinigrin was found significantly higher in reproductive part then the other plant parts. Concentration range of sinigrin in stem of thirty genotypes was from 9.4 µmol/g DM (Kranti) to 32.46 µmol/g DM (RH-8701). In leaves it ranged from 14 µmol/g DM (Varuna) to 64 µmol/g DM (B-85 glossy). As compare to vegetative parts in reproductive parts, sinigrin was found higher in concentration. It ranged from 31.02 µmol/g DM (Kranti) to 73.49 µmol/g DM (T-6342) in floral part.