Is L-buthionine sulfoximine reverse the benzimidazole resistance in Haemonchus contortus of small ruminants?

Abstract

Anthelmintic resistance (AR) in parasites of livestock is an emerging problem in many parts of the World. Hence, finding new classes of anthelmintic drugs or anthelmintic resistance reversal agents is the need of the hour to sustain the global livestock production. The aim of present study was to study the Benzimidazole (BZ) resistance reversal property of L-buthionine sulfoximine (L-BSO) using in vitro egg hatch assay (EHA). Thiabendazole (TBZ) alone and combination of TBZ and 200µM, 500µM and700µML-BSO were used with proper control and the hatching percentage and ED50values were recorded. It was found that significant (P 500µM) and the effect was dose dependent. Hence, L-BSO might potentially be used as a BZ resistance reversal agent to treat BZ resistant nematode parasites of livestock