Isolation and authentication of bacterial strains from root nodules of Sesbania aculeata in diverse agroecological zones of India

Abstract

Root nodules were collected from young and healthy seedling of Sesbania aculeata using trap plant method from the soil samples collected from field at different locations of India. Ten isolates were isolated from the root nodule of Sesbania aculeata and characterized by regular tests. All strains were gram-negative and did not absorb red color when cultured in YEMA containing congo red. In the ketolactose test yellowish zone of Cu2O not found. Moreover isolates have either deprived or no growth on the Hofer’s test which is representing nature of rhizobia. All isolates were fast grower which is confirmed by acid-alkaline production test.