In vitro studies in Arnebia hispidissima Arnebia hispidissima callus culture; the initiation, growth and organogenesis of callus derived from various explant sources

Abstract

Callus was obtained from internode segments, shoot meristem and axillary buds of Arnehia hispidissima. 2.5 mg/1 2,4-D containing MS medium proved to be the best among different concentration of 3 different growth regulators, used for callus induction. Shoot meristem showed significantly highest callusing (80%) with earliest callus initiation (8 days) among internode (75%, 10 days) and axillary bud (35%, 12 days). No callus could be obtained from leaf segments. Calli derived from these explants were successfully maintained by subculture on the same medium. After 25 days of callus induction, calli were transferred to MS medium supplemented with different growth regulators in 32 different combinations for regeneration. Highest regeneration was obtained on MS medium supplemented with 1.5 mg/1 kinetin + 2.5 mg/1 NAA in shoot meristem (79.2%) and axillary buds (32.5%), while highest regeneration (73.4%) was obtained on 2.0 mg/1 BAP with 0.5 mg/1 NAA containing MS medium in internode cultures.