Effects of Sub-culturing on In Vitro Shoot Multiplication in Banana Cultivar “Grand Naine”

Abstract

Present study describes the effects of sub-culturing on in vitro shoot multiplication in plantlets of banana cv. Grand Naine growing on basal MS and CHU media fortified with different concentrations of BAP and Kinetin. Maximum number of shoots was regenerated on full-strength basal MS or CHU medium when fortified with 2.0 mg/L BAP, whereas, the minimum number of shoots recorded on both culture media with 3.0 mg/L Kinetin. The results reported in the current study conclude that the successive behavior of in vitro shoot multiplication with the sub-culture cycles indicate the continuity of higher shoot multiplication rates at commercial point of view for banana cultivation.