Study of simple sequence repeat (SSR) polymorphism for Saltol QTL targeted at chr.1 in elite CMS IR 58025B of paddy and FL478

Abstract

Marker-assisted selection is an unequivocal translational research tool for crop improvement in the genomics era. IR 58025 B (25B) is an elite cytoplasmic male sterile line which widely accepted all over world for development hybrid paddy. Hybrid rice offers a wide opportunity to improve rice productivity in India. Hybrid rice has the potential to increase yields by 15% to 20% over those of conventionally bred varieties (Virmani, 1994). Most popular CMS source (IR 58025B) for rice hybrids sensitive to salinity. Here, we studied Single sequence repeat polymorphism in between a highly salt tolerant line FL478 and widely adopted salt sensitive CMS source IR 58025B for Saltol Qtl located at chromosome No.1. Saltol Qtl linked 50 markers served for targeted Qtl with both lines, these 50 SSR markers present in between RM 10710 to RM 10838 which is the peak marker for Saltol QTL present on carrier chromosome (Chr 1) There were ten foreground markers found polymorphic between rice cytoplasmic male sterile maintainer 58025B and Saltol Qtl donor FL478 at targeted Saltol QTL region on chromosome. These identified polymorphic markers are helpful to for introgression of Saltol Qtl in CMS source of paddy. Saltol Qtl introgressed CMS lines can play a pivotal role in success and sustenance of hybrid rice technology.