In vitro direct regeneration of Dalle Khursani (Capsicum annum) from salicylic acid treated explants

Abstract

A study was conducted with cotyledon, shoot tip and hypocotyl explants derived from Dalle Khursani; an important chilli cultivar of Sikkim to estimate the in vitro regeneration efficiency of salicylic acid treated explants. Explants were cultured on MS media supplemented with different combination of plant growth regulators along with 500 µM salicylic acid. All the three explants type showed response to the growth media, however the response was best observed on cotyledon explants with an average of 2.75 shoots per explant. MS medium supplemented with 4 mg/l TDZ (Thidiazuron) and 500 µM salicylic acid was found to be best for in vitro direct regeneration from all the three types of explants with an average of 2.93 shoots per explant. MS medium containing 2 mg/l GA3 (Giberrellic Acid 3) + 0.5 mg/l IAA (Indole Acetic Acid) was found to be suitable for both shoot elongation and rooting with mean shoot length of 3.10 ± 0.33 cm and 6.35 ± 0.98 roots per explant respectively.