Isolation, characterization of seconadry metabolites from the Melia azedarach Linn. Root and to evaluate their in vitro antidiabetic activity

Abstract

Objectives: The present study was to isolate, characterize from the Melia azedarach Linn. root and screen them for in-vitro antidiabetic activities. Method: The M. azedarach Linn. root coarse powder was subjected to extraction, fractionation and column chromatography to isolate the compounds. The identical eluents were collected and subjected to flash chromatography using Peak track software. The eluents were monitored on TLC and visualized under UV. The isolated compounds were characterized by IR, Mass and 1HNMR and 13CNMR spectra. Later, they were screened for in-vitro antidiabetic studies. Results: Three known compounds 1-methylacrylyl-3-acetyl-11-methoxymeliacapinin, Meliacarpinin B and Quercetin were isolated from Melia azedarach root extract. The spectroscopic data confirmed their structures similar to the reported compounds. From the in-vitro antidiabatic studies the isolated compounds exhibited I±-amylase, sucrase and I±-glucosidase inhibitory activities. Conclusion: We isolated three known compounds from root of melia azedarach Linn. and they exhibited significant in-vitro antidiabetic activities.