Investigation of in vivo analgesic and anti-inflammatory activities of methanol extracts of Phyllanthus reticulatus and Mimosa pigra

Abstract

The petroleum ether, ethyl acetate, and methanol extracts of Phyllanthus reticulatus. Poir. (Euphorbiaceae) were chosen for pharmacological screening. Comparative antioxidant, antibacterial activities and general toxicity studies on the n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of Mimosa pudicaand Mimosa rubicaulis, two Bangladeshi medicinal plants, were carried out, using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtitre plate based assay, and the brine shrimp lethality assay, respectively. The research work was conducted to investigate the in vivo Analgesic and Anti-inflammatory effects of Leaves of Phyllanthus reticulatus and Mimosa pigra. The Leaves of both plants extracted with Methanol (80%). The Analgesic activity of the extracts was performed using acetic acid induced Writhing in mice and Formalin induced to have Analgesic activity of the dose 100 and 200mg/kg body weight in the tested models compared to control. The percent of inhibition of standard drug and extract (100 and 200 mg/kg) was 40.19%, 51.95%, for Phyllanthus reticulatus and 44.73%, 55.75% for Mimosa pigra respectively. The Analgesic activity of the extract using Formalin induced Biting and Licking in mice was studied MELPR and MELMP were found to have Analgesic activity of the dose 100 and 200 mg/kg body weight in the tested models compared to control. The Anti-inflammatory activity of the extract using Carrageenan induced Anti-inflammatory in mice was studied of the dose 100 and 200 mg/kg body weight in the tested models compared to control. The crude extract of the plant were found to have Anti-inflammatory activity of the dose 100 and 200 mg/kg body weight in the tested models compared to control.So Methanolic extract of Phyllanthus reticulatus and Mimosa pigra have remarkable Analgesic and Anti-inflammatory activities.