Pathological and biochemical study on liver of male mice intoxicated with thioacetamide

Abstract

The present study is aimed to study the hepatotoxic effect of thioacetamide on male mice. This study was carried out at college of veterinary medicine, Baghdad from November 2016 to April 2017. One hundred twenty albino male mice were divided into two groups. First group was control. The second group divided into three subgroups. The first subgroup received thioacetamide intraperitoneally100 mg/kg for two months. The second subgroup received thioacetamide intraperitoneally 100 mg/kg for four months. The third subgroup received thioacetamide intraperitoneally 100 mg/kg for six months. All animals were euthanized and blood samples were taken for biochemical analysis and liver sample obtained and fixed in 10% normal buffer formalin for routine histopathological examination. Results showed significant increase in Alkaline phosphatase, Alanine Aminotanspherase, Aspartate Aminotranspherase and Bilirubin while showed significant decrease in Glutathione and peroxynitrate radicals concentration. Histopathological changes characterized by centrilobular necrosis, fibrosis, liver cirrhosis and hyperplasia of bile ducts.