A rapid In vitro propagation protocol of local germplasm of Bacopa monnieri (L.) induced through direct organogenesis from nodal explants

Abstract

The natural propagation of local germplasm of Bacopa is affected by poor seed viability, death of seedlings at two leaved stage, marshy areas requirements and slow growth of stem cutting. An efficient in vitro propagation protocol through direct organogenesis from nodal explants has been reported here for local germplasm of Bacopa monnieri. For axillary bud break and shoot proliferation from nodal segments, MS basal media supplemented with 1.0 mg/l BAP and 1.0 mg/l IAA, supported the best response (85% induction) and the same media combination also offered the best result for multiple shoot formation. The average number of shoots (10) and average shoot length (8.2 cm) was observed from nodal segment. Best performing rooting media (MS + 20g/l sucrose and 7g/l agar) induced rooting in 81% of shoots. The average number of roots (6) and average root length (3.1 cm) was observed from in vitro regenerated shoot. Two weeks after hardening plants were transferred to soil in field conditions and 90% survival rate was observed, and the growth of hardened plantlets was vigorous.