Immunological and pathological effect of lactoferrin against murine leishmaniasis

Abstract

The total numbers of animals in the experimental 40 mice of both sexes divided into 2 groups: The first group (30 mice) was immunized with lactoferrin orally with a dose of 0.25mg/10g (B.w.) in a volume of 0.1 ml daily for 10 days and then challenged with L. major. The 2nd group (10 mice) were non-immunized serve as a positive control. Then each group sacrificed by chloroform overdosing and blood was collected to measure serum IgG through by using ELISA Kit (mouse IgG Elisa Kite). The IgG levels were measured both in mice immunized with lactoferrin and in healthy controls and the results were then compared with each other. In our study, IgG levels were significantly higher in sera than in sera of the control group (P