Optimization of media for micro-propagation of Aloe barbadensis Mill.

Abstract

An efficient protocol for rapid in vitro propagation of valuable medicinal plant, Aloe barbadensis Mill. (var. Gujrat 20) by using shoot tip as explants were done. Aloe barbadensis is likely to become a major source of a number of medicinal products of high value in the coming future. Therefore, efficient and fast mechanized production need to be established for Aloe barbadensis to address the need of the present time. Our works deal with the establishment of a viable protocol for decontamination with HgCl2, in vitro shooting and rooting of this valued medicinal plant. Out of nine different hormonal regime tried for induction of multiplication on shoot explants, MS media supplemented with 6.0 mg/l BAP + 0.2 mg/l IAA + 100.0 mg/l AdSO4 was found best to induce maximum number of plantlets per explants. For rooting, increase in auxin (IAA) and decrease in cytokinin (BAP) and adenine sulphate was found effective.