Embryo excision and growth evaluation of chickpea (Cicer arietinum) on tissue culture medium

Abstract

In present study efforts were made to improve to study the effects of different growth regulators with different concentration on excised embryo from overnight soaked mature seeds of Chickpea. For study of Chickpea, seeds were sterilized and soaked overnight aseptically and then used for explants isolation. For both explants we use the mature Chickpea seeds. These seeds used for isolation of embryonic axis and Cotyladory Node explants. Both explants inoculated on different concentrations of growth regulators. Among those MS media fortified with 3 mg/L BAP shows maximum shoot initiation. The mature embryonic axis explants of cv. Vijay (Phule G-81-1-1) showed maximum growth and development in all types of media for all types growth conditions. The MS media fortified with 0.3 mg/L IBA as growth regulators gave better results for root formation in both types of explants and in both cultivars. In MS media without growth regulators, very poor response had occurred.