Characterization of phosphate solubilizing bacteria isolated from paddy rhizosphere of Vidarbha region

Abstract

In present investigation main aim to study morphology, biochemical characters and phosphate solubilising efficiency of phosphate solubilizing bacteria (PSB) from different soil samples were collected during June to October 2014. A total of forty-one (41) PSB were isolated from the eighty two (82) rhizospheric sample of rice plant of Vidarbha region, Maharashtra. Out of 41 isolates; 10 isolates show remarkable zone of solubilization. In vitro evaluation of phosphate solubilising ability of these ten (10) isolates were studied at department of plant pathology, Post Graduate Institute Dr. P.D.K.V., Akola during the June 2014 to May 2015. The zone of solubilization was studied on Pikovskaya’s (PVK) agar and quantitative phosphate solubilization was carried out by Vanado- molybdate method. PSB9 showed the maximum phosphate solubilization of 10.17 mm in PVK’s agar plates along with phosphate solubilizing activity (41.00 mg P2O5/100ml) in PVK’s broth and pH of the medium decreased up to 3.80. However, the isolate PSB-3 shows the least solubilizing activity. Potent isolates show good phosphate solubilizing ability and thus were found potential further used as bio fertilizer agents.