Production of Laccase by Ganoderma lucidium NCIM 1091 from agro-industrial by-product (Banana Peel)

Abstract

The present study investigated the synergistic effect of nutritional supplements and inducer (gallic acid) and amino acids on laccase production by Ganoderma lucidium NCIM 1091 from utilizing agro-industrial by-product (banana peel) under submerged fermentation conditions. Further optimization of parameters i.e. amount of substrate, concentration of amino acids and inducers for maximum laccase production was studied by central composite rotatable design. From the present study, it can be concluded that Ganoderma lucidium NCIM 1091 can be used to produce laccase enzyme. Banana peel was proved to be a good substrate for laccase production. Alanine and gallic acid proved to increase laccase production significantly. The interactive effect of amount of banana peel and concentration of amino acid and gallic acid was also proved to be significant at some levels and maximum laccase production (4.32 U/ml) was obtained at 8.63 gm banana peel, 50.23 mg/ml concentration of alanine and 0.25 mM concentration of gallic acid.