Characterization of zinc solubilization and organic acid detection in Pseudomonas sp. RZ1 from rice phyllosphere

Abstract

A number of sixty four bacterial isolates from rice phyllosphere region were screened for dissolution of zinc under in vitro studies. Among them the isolate RZ1 showed positive results for solubilization of zinc components was further characterized and identified into Pseduomonas sp. The isolate showed sharp decline in the pH of the growth medium after 12 hrs of incubation and the titratable acidity was measured as 1.44 mg/ml after 48 hrs of incubation. The culture filtrate of RZ1 isolate subjected to HPLC, GC-MS and LC-MS indicating the presence of acetic acid and gluconic acid in the extracellular supernatant.