Impact of Bacillus subtilis isolates on biochemical profile of Spodoptera litura (Fab.) larvae

Abstract

A laboratory experiment was carried out during 2014-2015 to study the effect of toxins of Bacillus subtilis isolates on the biochemical profile of newly ecdysed 3rd instar Spodoptera litura larvae fed for 24 h on castor oil bean leaves treated with 1000 and 2000 ppm concentrations by leaf dip and larval dip methods at Tamil Nadu Agricultural University, Coimbatore. There was a severe reduction in total protein content of larvae when treated with the isolate KA3 (55.24 mg g-1) followed by VB1 (56.90 mg g-1) at 2000 ppm by leaf dip method and total lipid content after 48 h was decreased (11.38 mg g-1, 12.33 mg g-1) in larvae treated with isolate VB1 at 1000 and 2000 ppm by the leaf dip method. Isolate VB1 showed higher GOT (Glutamic-oxaloacetic Transaminase) enzyme activity (24.43 units ml-1, 22.76 units ml-1) at 2000 ppm.