Study the pharmacokinetic of the Staphylococcus aureus specific bacteriophage in healthy animals

Abstract

The objective of the study was to investigate the pharmacokinetic of the Staphylococcus aureus specific bacteriophage in healthy animals first time in Iraq. This research were conducted from march to July, 2017. The current study includes two experiments. The first one was isolation of bacteriophage. It was separated from sewage samples by double layer technique: the isolated phage was categorized by rounded, clear plaques extended between 2-3 mm in size. While the second main experiment was studying the pharmacokinetics properties of the phage. The main parameters measured were the mean log PFU/ml of bacteriophage in blood plasma of rabbits after intravenous administration (IV) of 3 × 108 pfu/ml was (10.40 ± 0.11) after (0.08 hr.). The phage concentrations decreased to (1.30 ± 0.23) after (7 hr.). The pharmacokinetic study showed that phage was rabidly absorbed through gut wall and highly distributed.