Detecting of knock down resistance (Kdr) F1534C allele in the dengue vector Aedes aegypti in peri-urban areas of Colombo South, Sri Lanka

Abstract

A phenylalanine (F) to cysteine (C) substitution at position 1534 within the third domain of the NaV is associated with resistance to type I pyrethroid, permethrin, among populations of Aedes aegypti. Mosquito eggs were collected from three dengue high risk MOH areas in the Colombo district. Samples were reared to adulthood and resistance status against permethrin was determined by standard WHO susceptibility bioassays. Permethrin-resistant insects were genotyped for the F1534C mutation. All the PCR products were separated on 3.5% agarose gel and visualized by UV trans-illumination. Overall susceptibility was 58.3%, with mortality rates varying widely from 12.0% to 61.0%. Samples obtained from all three sampling sites showed high degrees of incipient resistance (