Biochemical characterization and biological control measures of citrus variegated chlorosis (CVC) bacteria

Abstract

The present investigation was undertaken to isolate and characterize citrus variegated chlorosis (CVC) bacteria as well as evaluation of its control measures through standard antibiotics and medicinally important plant extracts. The isolated bacteria was characterized by different morphological and biochemical test methods. Standard antibiotics and antibacterial sensitivity of plant extracts were tested against the isolated bacteria using disc diffusion method. In biochemical tests isolated bacteria was found to be positive in carbohydrate fermenting, catalase, Simmons Citrate, Methyl red, Kligler Iron Agar test, tween 80 test and potassium hydroxide test, while it showed urease test negative. The extract of Allium sativum showed highest antibacterial activity with 10±.5mm diameter of zone of inhibition. Erythromycin showed highest antibiotic activity with 32±0.5mm diameter of zone of inhibition against isolated bacteria. The present investigation would be helpful for further detection and biological management of CVC disease caused by Xylella fastidiosa pathogen.