Comparative Effect between Mesenchymal stem cells (MSCs) and Clomiphine citrate on induced premature ovarian failure (POF) in mice

Abstract

The present study was conducted to do a comparative between MSCs and Clomiphene citrate on induced POF in mice in College of Veterinary Medicine/ University of Baghdad from September 2016 to September 2017. 4-vinylcyclohexene diepoxide (80 mg/kg) B.W was used to induce POF. Forty females mice were separated into four groups, the first group (T1) was control, second group (T2) was induced and handled with phosphate buffer saline, third group (T3) was induced and handled with MSCS (25x 106cell / kg) B.W, fourth group (T4) was induced and treated with Clomiphine citrate (0.1 mg/kg) B.W. The results of T3 showed regulation in hormones with normalize the glutathione peroxides, superoxide dismutase levels, fertility index% with a significant increase in serum vascular endothelium growth factor as compared with T2 and T4. Histopathological result of T3 showed regular outline of growing ovarian follicles as compared with T1, T2 and T4. This study concluded that the MSCs had inhibitory effect to POF without side effect whereas Clomiphene citrate was attributed in healing of POF with many side effects.